continuous flow chamber Search Results


90
AVESTIN Inc continuous-flow chambers with polycarbonate filters
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow Chambers With Polycarbonate Filters, supplied by AVESTIN Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous-flow chambers with polycarbonate filters/product/AVESTIN Inc
Average 90 stars, based on 1 article reviews
continuous-flow chambers with polycarbonate filters - by Bioz Stars, 2026-06
90/100 stars
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90
KU Leuven continuous flow chambers
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow Chambers, supplied by KU Leuven, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous flow chambers/product/KU Leuven
Average 90 stars, based on 1 article reviews
continuous flow chambers - by Bioz Stars, 2026-06
90/100 stars
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90
BioSurface Technologies Corporation continuous-flow chamber
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow Chamber, supplied by BioSurface Technologies Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous-flow chamber/product/BioSurface Technologies Corporation
Average 90 stars, based on 1 article reviews
continuous-flow chamber - by Bioz Stars, 2026-06
90/100 stars
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90
PermeGear Inc continuous flow temperature-controlled perfusion chambers
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow Temperature Controlled Perfusion Chambers, supplied by PermeGear Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous flow temperature-controlled perfusion chambers/product/PermeGear Inc
Average 90 stars, based on 1 article reviews
continuous flow temperature-controlled perfusion chambers - by Bioz Stars, 2026-06
90/100 stars
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90
Bronwill Scientific Inc continuous flow/high intensity chamber
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow/High Intensity Chamber, supplied by Bronwill Scientific Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous flow/high intensity chamber/product/Bronwill Scientific Inc
Average 90 stars, based on 1 article reviews
continuous flow/high intensity chamber - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).

Journal: ACS Synthetic Biology

Article Title: ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis

doi: 10.1021/acssynbio.2c00075

Figure Lengend Snippet: ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).

Article Snippet: The continuous-flow chambers with 50 nm polycarbonate filters (Avestin) were preequilibrated with 50 mM KPi pH 7.0.

Techniques: Liquid Chromatography with Mass Spectroscopy