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AVESTIN Inc
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KU Leuven
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BioSurface Technologies Corporation
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PermeGear Inc
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Bronwill Scientific Inc
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Image Search Results
Journal: ACS Synthetic Biology
Article Title: ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis
doi: 10.1021/acssynbio.2c00075
Figure Lengend Snippet: ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Article Snippet: The continuous-flow chambers with 50 nm
Techniques: Liquid Chromatography with Mass Spectroscopy